Anti-EPO-R; clone VP-2E8

Code: MABS1287 D2-231

Application

Research CategorySignaling

Anti-EPO-R, clone VP-2E8, Cat. No. MABS1287, is a mouse monoclonal antibody that detects erythropoietin receptor and is tested fo...


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Application

Research CategorySignaling

Anti-EPO-R, clone VP-2E8, Cat. No. MABS1287, is a mouse monoclonal antibody that detects erythropoietin receptor and is tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Immunoprecipitation.

Flow Cytometry Analysis: 2 µg/mL from a representative lot detected EPO-R in UT-7 (EPO dependent) cells.

Immunoprecipitation Analysis: A representative lot detected EPO-R in Immunoprecipitation applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Immunofluorescence Analysis: A representative lot detected EPO-R in Immunofluorescence applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Flow Cytometry Analysis: A representative lot detected EPO-R in Flow Cytometry applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Erythropoietin receptor (UniProt: P19235, also known as EPO-R) is encoded by the EPOR gene (Gene ID: 2057) in human. EPO-R is a single-pass type I membrane protein that serves as a receptor for erythropoietin. It mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPO-R dimerizes triggering the JAK2/STAT5 signaling cascade. EPO-R has an extracellular domain (aa 25-250), a transmembrane domain (aa 251-273), and a cytoplasmic domain (aa 274-508). It is synthesized with a signal peptide (aa 1-24) that is cleaved to generate mature EPO-R. Upon stimulation following Erythropoietin (EPO) binding, EPO-R is phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins, which mediate cell proliferation. Phosphorylation on Tyrosine 454 is shown to be essential for PTPN6 interaction, and on Tyrosine 426 for PTPN11 interaction. Tyrosine 426 is also required for SOCS3 binding, however, Tyr-454/Tyr-456 motif is reported to be the preferred binding site. Ubiquitination of EPO-R at Lysine 81 is required for receptor internalization, whereas ubiquitination at Lysine 453 promotes trafficking of activated receptors to the lysosomes for degradation. Defects in EPOR gene are linked to familial erythrocytosis which is characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, and hypersensitivity of erythroid progenitors to erythropoietin.

Immunogen

A recombinant fragment corresponding to the extracellular domain of human EPO-R.

Epitope: extracellular domain

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Quality

Evaluated by Immunocytochemistry in starved UT-7 cells.

Immunocytochemistry Analysis: 1:500 dilution of this antibody detected EPO-R in starved UT-7 cells.

Specificity

Clone VP-2E8 recognizes EPO-R in human cells. It targets an epitope within the extracellular domain.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

55.06 kDa calculated.

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcemouse
cloneVP-2E8, monoclonal
Gene Informationhuman ... EPOR(2057)
isotypeIgG1κ
NCBI accession no.NP_000112
packagingantibody small pack of 25 µg
Quality Level100
shipped inambient
species reactivityhuman
technique(s)immunocytochemistry: suitable, flow cytometry: suitable, immunoprecipitation (IP): suitable, immunofluorescence: suitable
UniProt accession no.P19235
This product has met the following criteria to qualify for the following awards:



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